Release kinetics of actives from chewing gums into saliva monitored by direct analysis in real time mass spectrometry

Authors: Nicolas Jeckelmann, Olivier P. Haefliger
Rapid Communications in Mass Spectrometry 2010, Apr, 30, 24(8), 1165-1171
DOI: 10.1002/rcm.4500

 

Direct analysis in real time mass spectrometry (DART®-MS) was used to
monitor the release kinetics of a taste-refreshing compound from
chewing gums into the saliva of subjects. A new DART®-MS sample probe
was designed which was about four times more sensitive than the
current benchmark probe. This decreased the impact of the dilution of
the saliva samples that was required to minimize ion suppression
effects and make quantitative analyses without an internal standard
possible. The new probe was also about three times more reproducible,
which allowed quantitative measurements to be conducted manually
without requiring the enhanced precision provided by an automatic
sample positioner. The accuracy of analyses performed by DART®-MS was
verified by comparing the results obtained from saliva samples
analyzed both by DART®-MS and by a more classical liquid chromatography/
mass spectrometry (LC/MS) method. This investigation showed good
agreement between the two techniques. DART®-MS could then be used to
objectively demonstrate the efficiency of a granular carbohydrate-
based delivery system to boost for a few minutes the release of a
lipophilic flavor raw material with a high octanol/water partition
coefficient, cyclohexanecarboxamide, N-ethyl-5-methyl-2-(1-
methylethyl) (WS-3), from chewing gum into saliva.